Специфичность встраиваний P элемента в локус генов бтш70 D. melanogaster и влияние инсерций на функционирование этих генов
Диссертация
В промоторных областях были зарегистрированы «горячие точки» встраиваний конструкции EPgy2 — положения -96, -97 от старта транскрипции генов бтшЮ, что связано с особенностями структуры этой области промотора. Проведены эксперименты по исследованию влияния инсерций в гены бтшЮ на уровне целого организма: обнаружена лучшая выживаемость мух при средних ТШ и незначительная задержка развития… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Белки и гены теплового шока: общая характеристика
- 1. 1. 1. Гены теплового шока
- 1. 1. 2. Основные семейства БТШ
- 1. 1. 3. Механизмы регуляции ответа на тепловой шок
- 1. 2. Структура кластера генов бтш70 у Diptera
- 1. 3. Мобильные элементы как возможный фактор эволюции генов ТШ
- 1. 4. Особенности Р элемента и его использование в инсерционном мутагенезе
- 1. 1. Белки и гены теплового шока: общая характеристика
- 2. МАТЕРИАЛЫ И МЕТОДЫ
- 2. 1. Линии Drosophila
- 2. 2. Модель генетического анализа
- 2. 3. Выделение геномной ДНК из мух
- 2. 4. Гибридизация геномной ДНК по Саузерну
- 2. 5. ПЦР-анализ
- 2. 6. Выделение фрагментов ДНК
- 2. 7. Секвенирование ДНК
- 2. 8. Количественный ПЦР-анализ в реальном времени (qRT-PCR) 43 2.9 Выделение мРНК из мух и ее анализ методами электрофореза и Нозерн-гибридизации
- 2. 10. Лигирование фрагментов ДНК
- 2. 11. Трансформация компетентных клеток Е. col
- 2. 12. Выделение плазмидной ДНК
- 2. 13. Одномерный электрофорез белков
- 2. 14. Вестерн-блоттинг
- 2. 15. Двумерный электрофорез белков
- 2. 16. Включение метионина- S в белки слюнных желез Drosophila
- 2. 17. Определение термоустойчивости мух
- 2. 18. Определение фертильности трансгенных мух 48 2.19 Иммунофлуоресцентное окрашивание транскрипционых факторов 48 2.20. Статистическая обработка данных
- 3. РЕЗУЛЬТАТЫ И ИХ ОБСУЖДЕНИЕ
- 3. 1. Характеристика системы скрещиваний для получения инсерций конструкции на основе Р элемента в гены бтшЮ методом Р-инсерционного мутагенеза
- 3. 2. Идентификация инсерций EPgy2 методом Саузерн-блот анализа 53 3.3 Точная локализация полученных инсерций EPgy
- 3. 4. Влияние инсерций EPgy2 на уровень транскрипции бтшЮ
- 3. 5. Анализ синтеза БТШ70 в трансгенных линиях
- 3. 6. Анализ инсерций EPgy2, сопровождающихся перестройками геномной ДНК
- 3. 7. Базальная и индуцированная термоустойчивость трансгенных линий
- 3. 8. Определение плодовитости потомства трансгенных линий
- 3. 9. Вклад нмБТШ в термоустойчивость
- 4. ВЫВОДЫ
Список литературы
- Васильева Л.А., Ратнер В. А., Бубенщикова Е. В. Стрессовая индукция транспозиций ретротранспозонов дрозофилы: реальность явления, характерные особенности и возможная роль в быстрой эволюции// Генетика, 1997. Т 33(8), С1083−1093
- Гарбуз Д.Г., Молодцов В. Б., Великодворская В. В., Евгеньев М. Б., Зацепина О. Г. Эволюция ответа на тепловой шок внутри рода Drosophila! I Генетика, 2002. Т 38(8) С 1097- 1109
- Гусев Н.Б., Богачева Н. В., Марстон С. Б. Структура и свойства малых белков теплового шока (slisp) и их взаимодействие с белками цитоскелета// Биохимия, 2002. Т 67 (5) С 613−623
- Евгеньев М. Б., Шейнкер В. Ш., Левин А. В. Молекулярные механизмы адаптации к гипертермии у высших организмов. I. Синтез белков теплового шока в клетках культуры различных видов шелкопряда и гусеницах. // Молекулярная биология, 1987. Т 21, С 484 -494
- Евгеньев М. Б., Мнджоян Е. И., Зеленцова Е. С., Шостак Н. Г., Лёзин Г. Т., Великодворская В. В., Полуэктова Е. В. Мобильные элементы и видообразование// Молекулярная биология, 1998. Т 32(1), С 184−192
- Лозовская Е. Р., Евгеньев М. Б. Тепловой шок у дрозофилы и регуляция активности генома// Молекулярная биология, 1984. Т 20, С 142 185
- Маргулис Б.А., Гужова И. В. Белки стресса в эукариотической клетке// Цитология, 2000. Т 42(4), С 323 342
- Ульмасов X. А., Каррыева Б. Ч., Караев К. Стрессовые белки и адаптация// Ашхабад, «Ылым», 1993.212с.
- Фаддеев М.А. Элементарная обработка результатов эксперимента: Учебное пособие. //Изд-во Нижегородского госуниверситета, 2002. 108 с.
- Хлебодарова ТМ. Как клетки защищаются от стресса?// Генетика, 2002. Т 38(4), С 437−452
- Шилова В.Ю., Гарбуз Д. Г., Евгеньев М. Б., Зацепина О. Г. Низкомолекулярные белки теплового шока и адаптация к гипертермии у разных видов Drosophilall Молекулярная биология, 2006. Т 40(2), С 271−276
- Aguilar-Mahecha A, Hales BF, Robaire В. Expression of stress response genes in germ cells during spermatogenesis // Biol Reprod. 2001- V 65(1), P 119−27
- Anxolabehere D. P transposable element in Drosophila melanogaster. horizontal transfer // С R Seances Soc Biol Fil., 1992. V 186(6) P 641−55
- Arhipova I.R., Lyubomirskaya N.V., Ilyin Y.V. Drosophila retrotransposones// Austin, Texas, 1995: R.G. Landes company. 134 p.
- Ayme A, Tissieres A. Locus 67B of Drosophila melanogaster contains seven, not four, closely related heat shock genes // EMBO J., 1985 T 4, VI1, P 2949 2954
- Bailey J.A., G. Liu and E.E. Eichler. An Alu transposition model for the origin and expansion of human segmental duplications// American Journal of Human Genetics, 2003. V 73, P 823−834
- Balakrishnan K., A. De Maio. hsp70 binds it’s own messenger RNA as part of gene expression self-limiting mechanism // Cell stress& Chaperones, 2006. VI1 (1)
- Becker J., and E. A. Craig. Heat-shock proteins as molecular chaperones// European Journal of Biochemistry, 1994. V 219, P 11−23
- Beckmann Richard P., Lovett Michelle, William J. Welch. Examining the Function and Regulation of HSP70 in Cell Subjected to Metabolic Stress// The Journal of Cellular Biology, 1992. T117(6), P 1137−1150
- Bellen H., C. J. O’Kane, C. Wilson, U. Grossniklaus, R.K. Pearson, W.J. Gehring. P-element mediated enhancer detection: a versatile method to study development in Drosophila! I Genes Dev., 1989. V 3, P 1288−1300
- Bettencourt BR, Feder ME. Hsp70 duplication in the Drosophila melanogaster species group: how and when did two become five?// Mol Biol Evol., 2001. T 18, V7, P 1272 1282
- Bettencourt B.R., and M.E. Feder 2002a. Rapid concerted evolution via gene conversion at the Drosophila hsp70 genes // Journal of Molecular Evolution, V 54, P 569−586
- Bettencourt B. R., I. Kim A., A. Hoffmann and M, E. Feder, 20 026. Response to natural and laboratory selection at the Drosophila hsp70 genes// Evolution, V 56, P 1796−1801
- Bienz M., H.Pelham. heat shock regulatory elements function as an inducible enhancern in the Xenopus hsplO gene and when United to a heterologous promoter// Cell, 1986. V 45, P 753 -760
- Bingam P.M., Kidwell M.G., Rubin G.M. The molecular basis of P-M hibrid dysgenesis: the role of the P-element, P-strain specific transposon family. // Cell, 1982. V 29, P 995−1004
- Boutanaev A. M., A. I. Kalmykova, Y. Y. Shevelyou and D. I. Nurminsky. Large clusters of co-expressed genes in the Drosophila genome! Nature, 2002. V 420, P 666−669
- Bucheton A., Busseau I., Teninges D. I elements in Drosophila melanogaster! I Mobile DNAII, 2002, P. 796−812
- Bush KT, Goldberg AL, Nigam SK. Proteasome inhibition leads to a heat-shock response, induction of endoplasmic reticulum chaperones, and thermotolerance// J. Biol Chem., 1997. V 272(14), P 9086−92
- Busseau I.A., Pelisson A., Bucheton A. I elements of Drosophila melanogaster generate specific chromosomal rearrangements during transposition// Mol. Gen. Genet., 1989. V 218 (2) P 222−228
- Cervera J. Induction of self-tolerance and enhanced stress protein synthesis in L-132 cells by cadmium chloride and by hyperthermia// Cell Biol Int Rep., 1985. V 9(2), P131−41
- Chen Q, Ma E, Behar KL, Xu T, Haddad GG. Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster! I J. Biol. Chem., 2002. T 277, V5, P3274 3279
- Chomczynski P. and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction// Anal. Biochem., 1987. V 162, P 156−159
- Chun-Yang Fan et al. Mechanisms for regulation of hsplO function by hspAO// Cell stress&Chaperones, 2003. V 8 (4), P 309−316
- Clark Jonathan B, Pyong C. Kim, and Margaret G. Kidwell Molecular Evolution of P Transposable Elements in the Genus Drosophila III. The melanogaster Species Group //Mol. Biol. Evol., 1998. V 15(6), P 746−755
- Cooley L, Kelley R, Spradling A Insertional mutagenesis of the Drosophila genome with single P elements//Science, 1988. V 239(4844), P 1121−8
- Corces V., Holmgren R., Freund R., Morimoto R. and Meselson M. Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B// Proc. Nati. Acad. Sci. USA, 1980. V 77(9), P 5390−5393
- Coveny Angela M, 1 Tammy Dray 1,2 and Gregory B. Gloor3The Effect of Heterologous Insertions on Gene Conversion in Mitotically Dividing Cells in Drosophila melanogaster //Genetics, 2002. V 161, P 249−258
- Daborn P. J, J. L. Yen, M. R. Bogwitz, G. Le Goff, E. Feil et al. A single P450 allele associated with insecticide resistance in Drosophila! I Science, 2002. V 297, P 2253−2256
- Daniels SB, Chovnick A P element transposition in Drosophila melanogaster. an analysis of sister-chromatid pairs and the formation of intragenic secondary insertions during meiosis.//Genetics, 1993. V 133(3), P 623−36
- Delmas F, Trocheris V, Murat J.-C. Expression of stress proteins in cultured HT29 human cell-line- a model for studing environmental aggression// Int. J. Biochem. Cell Biol, 1995. V 27, P 385 391
- Dix DJ. HsplO expression and function during gametogenesis// Cell Stress Chaperones, 1997. V2(2), P.73−77
- Dooha Kim et al. A Constitutive Heat Shock Element-binding Factor Is Immunologically Identical to the Ku Autoantigen.// The Journal of Biological Chemistry, 1995. V 270(25), P 15 277- 15 284
- Dreher D, Vargas J. R, Hochstrasser D, Junod A.F. Effects of oxidative stress and Ca2+ antagonists on molecular chaperones in human umbilical vein endothelial cells// Electrophoresis, 1995. V 16(7), P 1205−14
- Drnevich JM, Reedy MM, Ruedi EA, Rodriguez-Zas S and Hughes KA. Quantitative evolutionary genomics: differential gene expression and male reproductive success in Drosophila melanogaster! Proc. R. Soc. Lond, 2004. P 2267−2273
- Evgen’ev MB., Zelentsova H., Mnjoian L., Poluectova H., Kigwell M.G. Invasion of Drosophila virilis by the Penelope transposable element// Chromosoma, 2000. V 109, P 350 -357
- Evgen’ev MB, Kolchinski A, Levin A, Preobrazhenskaya AL, Sarkisova E. Heat-shock DNA homology in distantly related species of Drosophilall Chromosoma, 1978. T 68(4), P 357 -365
- Evgen’ev MB., Zatsepina OG., Garbuz D, Lerman D., Velikodvorskaya V, Zelentsova E, Feder ME. Evolution and arrangement of the hsplO gene cluster in two closely related species of the virilis group of Drosophila //Chromosoma, 2004. V 113(5), P 223−32
- Farkas G. et al. Chromatin organization and transcriptional control of gene expression in Drosophilall Gene, 2003. T 253, P 117−136
- Feder M. E. and Hofmann G. E. Heat-Shock Proteins, Molecular Chaperones, and the Stress Response, Evolutionary and Ecological Phisiology // Annual Review of Physiology, 1999. T 61, P 243−282
- Feder ME, Hofmann GE. 1998. Evolutionary and ecological physiology of heat-shock proteins and the heat-shock response: a comprehensive bibliography, http ://www. annure v. org/sup/material .htm
- Feder ME, Blair N, Figueras H. Natural thermal stress and heat-shock protein expression in Drosophila larvae and pupae// Funct. Ecol., 1997. V 11, P 90−100
- Fernandes M, Xiao H, Lis JT. Fine structure analyses of the Drosophila and Saccharomyces heat shock factor-heat shock element interactions// Nucleic Acids Res., 1994. V 22, P 167−73
- Frydman Judith. Folding of newly translated proteins in vivo: the role of molecular chaperones// Annu. Rev. Biochem, 2001. T 70, P 603 649
- Gabai V.L., Sherma M.Y. Molecular biology of thermoregulation: interplay between molecular chaperones and signaling pathways in survival of heat shock// J. Appl. Physiol, 2002. V 92, P 1743−1748
- Gebauer Mathias, Matthias Zeiner and Ulrich Gehring. Interference between proteins Hap46 and Hop/p60, which bind to different domains of the molecular chaperone hsplQ/hsclQH Mol. Cell. Biol., 1998. V 18 (11), P 6238 6244
- Georgel P.T. Chromatin potentiation of the hsplO promoter is linked to GAGA-factor recruitment // Biochem. Cell Biol., 2005. V 83, P 555−565
- Georgiev GP. Mobile genetic elements in animal cells and their biological significance//Eur J Biochem., 1984. V 145(2), P 203−20
- Gilmour D. S., G. H. Thomas and S. C. Elgin. Drosophila nuclear proteins bind to regions of alternating С and T residues in gene promoters// Science, 1989. V 245, P 1487−1490
- Gloor Gregory В., Jessica Moretti, Joanne Mouyall and Katherine J. Keeler2Distinct P-Element Excision Products in Somatic and Germline Cells of Drosophila melanogaster// Genetics, 2000. V 155, P 1821−1830
- Golovnin A, Georgieva S, Hovhannisyan H, Barseguyan K, Georgiev P. P element-mediated duplications of genomic regions in Drosophila melanogaster 11 Chromosoma, 2002. V 111(2), P 126−138
- Gong W. J., and K. G. Golic Genomic deletions of the Drosphila melanogaster HsplQ genes// Genetics, 2004. V 168, P 1467−1476
- Hahn JS, Hu Z, Thiele DJ, Iyer VR. Genome-Wide Analysis of the Biology of Stress Responses through Heat Shock Transcription Factor// Mol Cell Biol, 2004. V 24(12), P 52 495 256
- Hartl D. L, Lohe A. R, Lozovskaya E.R. Regulation of the transposable element mariner// Genetica, 1997. V 100, P 177−184
- Jachansan Amin, R. Nestril, P. Schiller, M. Dreano, R. Voellmy. Organization of the Drosophila melanogaster hsplO regulation unit// Mol. Cell. Biol, 1987. V 7(3), P 1055 1062
- Jachansan Amin, Jayakumar Ananthan, R. Voellmy. Key features of Heat Shock Regulatory Elements// Molecular and Cell Biology, 1988. V 8(9), P 3761 3769
- Kapitonov V. V., and J. Jurka Molecular paleontology of transposable elements in the Drosophila melanogaster genome// Proceedings of the National Academy of Sciences of the United States of America, 2003. V 100, P 6569−6574
- Karpov V. L., О. V. Preobrazhenskaya and A. D. Mirzabekov. Chromatin structure of hsplO genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region// Cell, 1984. V 36, P 423−431
- Kazazian H.H. Mobile elements: Drivers of genome evolution// Science, 2004. V 303, P 1626−1632
- Kidwell M. G., Damon R. Lisch Transposable elements as sources of genomic variation// in Mobile DNAII, edited by N.L. Craig et. al., 2002. ASM Press, Washington
- Kidwell M. G., Kidwell J. F., Sved J.A. Hybrid dysgenesis in Drosophila melanogaster. a syndrome of abberant traits including mutation, sterility and male recombination// Genetics, 1977. V 36, P 813−833
- Kidwell M. G. The evolutionary history of the P family of transposable elements// J. Heredity, 1994. V 85, P 339−346
- Kidwell M. G. Hybrid dysgenesis in Drosophila melanogaster. The relationship between the P-M and I-R interaction systems// Genet. Res., 1979. V 33, P 105−117
- Konstantopoulou I, Nikolaidis N, Scouras ZG. The hsplO locus of Drosophila auraria (montium subgroup) is single and contains copies in a conserved arrangement// Chromosoma, 1998. T 107, V 8, P 577−586
- Mccollum A., E. Ganko, P. Barrass, J. Rodriguez and J. Mcdonald. Evidence for the adaptive significance of an LTR retrotransposon sequence in a Drosophila heterochromatic gene// BMC Evolutionary Biology, 2002, 2:5
- McGarry, Т. J. and Lindquist, S. The preferential translation of Drosophila hsplO mRNA requires sequences in the untranslated leader// Cell, 1985. V 42, P 903−911
- Marchler G, Wu C. Modulation of Drosophila heat shock transcription factor activity by the molecular chaperone DROJ1 //EMBO J, 2001. V 20(3), P 499−509
- Marsano, R. M, R. Caizzi, R. Moschetti and N. Junakovic. Evidence for a functional interaction between the Baril transposable element and the cytochrome P450 cypl2a4 gene in Drosophila melanogaster 11 Gene, 2005. V 357, P 122−128
- Maside X, Bartolome C, Charlesworth B. S-element insertions are associated with the evolution of the HsplO genes in Drosophila melanogaster/У Curr. Biol, 2002. T 12, V 19, P 1686- 1691
- Mehlen P, Schulze-Osthoff K, Arrigo A.P. Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 blocks Fas/APO-1- and staurosporine-induced cell death// J Biol Chem, 1996. V 271(28), P 16 510−16 514
- Michaud S, Marin R, Westwood JT, Tanguay RM. Cell-specific expression and heat-shock induction of Hsps during spermatogenesis in Drosophila melanogaster/ /J Cell Sci, 1997. V 110, P 1989−97
- Molecular Chaperones and the Heat Shock Response. Cold Spring Harbor, New York, 1998. 299p.
- Morimoto Richard, Tissieres Alfred, Georgopoulos Costa. The Stress Response, Function of the Proteins, and Perspectives// Stress Proteins in Biology and Medicine, Cold Spring Harbor Laboratory Press, 1990. P. 1 32
- Morrow G, Heikkila JJ, Tanguay RM. Differences in the chaperone-like activities of the four main small heat shock proteins of Drosophila melanogaslerll Cell Stress Chaperones, 2006.1. V 11(1), P 51−60
- Nacheva, G. A., D. Y. Guschin, О. V. Preobrazhenskaya, V. L. Karpov, К. K. Ebralidze et al. Change in the pattern of histone binding to DNA upon transcriptional activation // Cell 1989.1. V 58, V 27−36
- Nakai, M.Tanabe. HSF4, a new member of the human Heat Shock Factor family which lacks properties of a transcriptional activator// Molecular and cellular biology, 1997. VI. P. 469 -481.
- Nassif N W.R. Engels DNA homology requirements for mitotic gap repair in Drosophilall Proc. Nati. Acad. Sci. USA, 1993. V 90, P 1262−1266
- Nassif N, J. Penney, S. Pal, W.R. Engels and G.B. Gloor. Efficient Copying of Nonhomologous Sequences from Ectopic Sites via P-Element-Induced Gap Repair// Mol and Cell Biol., 1994. V 14 (3), P 1613−1625
- Nelson R. John, Thomas Ziegelhoffer, Charies Nicolet, Margaret Wemer-Washburne, and Elizabeth A. Craig. The Translation Machinery and 70kd Heat Shock Protein Cooperate in Protein Synthesis// Cell, 1992. V 71, P 97 105
- Neupert Walter, Franz-Ulrich Hartl, Elizabeth A. Craig, and Nikolaus Pfanner. How Do Polypeptides Cross the Mitochondrial Membranes? // Cell, 1990. V 63, P 447 450
- Norris Carol E., Philip J. dilorio, R. Jack Schultz, and Lawrence E. Hightower. Variation in Heat Shock Proteins within Tropical and Desert Species of Poeciliid Fishes// Mol. Biol. Evol., 1995. V 12(6), P 1048- 1062
- O’Brien, Lis J.T. RNA polymerase II pauses at the 5'-end of the transcriptionally induced Drosophila hsp70 genet I Mol. Cell. Biol., 1991. V 11 (10), P 5285−5290
- O’Brien Т., R. C. Wilkins, C. Giardina and J. T. Lis. Distribution of GAGA protein on Drosophila genes in vivo// Genes & Dev., 1995. V 9, P 1098−1110
- O’Farrell P.H. High resolution two-dimentional electrophoresis of proteins.// J. Biol. Chem., 1975. V 250, P 4007−4021
- O’hare, K., and G. M. Rubin. Structures of P-Transposable Elements and Their Sites of Insertion and Excision in the Drosophila melanogaster Genome.// Cell, 1983. V 34, P 25−35
- Ohno A., Miller E., Fraek M., Rucker S., Beck F.-x., Thurau K. Ketoconazole inhibits organic osmolyte efflux and induces heat shock proteins in rat renal medulla. 1996// Kidney Intern. 50: 110- 118
- Patriarka E. J., Maresca B. Acquired thermotolerance following hsp synthesis prevents impairment in mithohondria ATP-ase activity at elevated temperatures in Saccharomyces cerevisiae// Exp. Cell. Res., 1990. V 190, P 57−64
- Petrov D., Schutzman J., Hartl D., Losovskaya E.R. Diverse transposable elements are mobilized in hybrid dysgenesis in Drosophila virilis// Proc. Natl. Acad. Sci. USA, 1995. V 92, P 8050−8054
- Pile Lori A. and David A. Wassarman Localizing transcription factors on chromatin by immunofluorescence // Methods, 2002. V 26, P 3 9
- Pinsker W, Haring E, Hagemann S, Miller WJ. The evolutionary life history of P transposons: from horizontal invaders to domesticated neogenes // Chromosoma, 2001. V 110 (3), P 148−58
- Quesneville H, Anxolabehere D. A simulation of P element horizontal transfer in Drosophila //Genetica, 1997. V 100(1−3), P 295−307
- Rabindran К Sridhar, Haroun J Raymond, Clos Joachim, Wisnievski Jan. Regulation of Heat Shock Factor trimer formaion: role of the conserved leucine zipper// Science, 1993. T 259, P 230−234
- Rio D.C. P Transposable Elements in Drosophila melanogaster! I Mobile DNA II, 2002, chapter 21. P 484- 518
- Robertson Hugh M, Christine R. Preston, Randall W. Phillis, Dena M. Johnson-Schlitz, Wendy K. Bern and William R. Engels. A Stable Genomic Source of P Element Transposase in Drosophila melanogaster// Genetics, 1988. V 118, P 461−470
- Rubin DM, Mehta AD, Zhu J, Shoham S, Chen X, Wells QR, Palter KB. Genomic structure and sequence analysis of Drosophila melanogaster HSC70 genes// Gene, 1993. T 128, V 2, P 155−163
- Russnak RH, Candido EP. Locus encoding a family of small heat shock genes in Caenorhabditis elegans: two genes duplicated to form a 3.8-kilobase inverted repeat// Mol. Cell. Biol, 1985. T 5, V 6, P 1268- 1278
- Sambrook J, Frisch EF, Maniatis T. Molecular cloning. A laboratory manual. 1989// New York, Cold Spring Harbor Press
- Sanchez Y, Taulien J, K. A. Borkovich and S. Linquist. Hspl04 is required for tolerance to many forms of stress// The EMBO journal, 1992. V 11 (6), P 2357 2364
- Sanchez Y, Parsell DA, Taulien J, Vogel JL, Craig EA, Lindquist S. Genetic evidence for a functional relationship between Hspl04 and Hsp70// J Bacteriol, 1993. V 175 (20), P 64 846 491
- Schlenke, T. A, and D. J. Begun. Strong selective sweep associated with a transposon insertion in Drosophila simulans //Proceedings of the National Academy of Sciences of the United States of America, 2004. V 101, P 1626−1631
- Schirmer EC, Glover JR., Singer MA, LindquistS. HSP100/Clp proteins: a common mechanism explains diverse functions// Trends Biochem Sci., 1996. V 21(8), P 289 296
- Shopland L.S., Hirayoshi K., Fernandes M., Lis J.T. HSF access to heat shock elements in vivo depends critically on promoter architecture defined by GAGA-factor, TFIID, and RNA-polymerase II binding sites// Genes Dev., 1995. V 9 (22), P 2756−2769
- Silbermann R and Tatar M. Reproductive costs of heat shock protein in transgenic Drosophila melanogaster I I Evolution, 2000. V 54, P 2038−2045
- Spradling, A. C., D. Stern, A. Beaton, E. J. Rhem, T. Laverty et al. The Berkeley Drosophila Genome Project gene disruption project: single P-element insertions mutating 25% of vital Drosophila genes// Genetics, 1999. V 153, P 135−177
- Terlecky S. R., Chiang H. L., Olson T. S., Dice J. F. Protein and Peptide Binding and Stimulation of In Vitro Lysosomal Proteolysis by the 73-kDa Heat Shock Cognate Protein// The Journal of Biological Chemistry, 1992. T 267, V 13, P 9202 9209
- Timakov В., X. Liu, I. Turgut and P. Zhang Timing and Targeting of P-Element Local Transposition in the Male Germline Cells of Drosophila melanogaster! I Genetics, 2002. V 160, P 1011−1022
- Tower, J., G. H. Karpen, N. Craig and A. C. Spradling. Preferential transposition of Drosophila P elements to nearby chromosomal sites.// Genetics, 1993. V 133, P 347−359
- Tsukiyama, Т., P. B. Becker and C. Wu. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor //Nature, 1994. V 367, P 525−532
- Tsuno K., Yamaguchi O. Chromosomal rearrangement In (2)TY and linkage maps of the second chromosome of Drosophila virilis! Jpn. J. Genet., 1991. V 66, P 49−58
- Uma S, Thulasiraman V, Matts R. L. Dual Role for HSC70 in the Biogenesis and Regulation of the Heme-regulated Kinase of the Alpha Subunit of Eukaryotic Translation Initiation Factor 111 Molecular and Cellular Biology, 1999. T 19, V 9, P 5861 5871
- Udvardy, A, E. Maine and P. Schedl. The 87A7 chromomere. Identification of novel chromatin structures flanking the heat shock locus that may define the boundaries of higher order domains //J Mol Biol, 1985. V 185, P 341−358
- Ulmasov KA, Shammakov S, Karavaev K, Evgen’ev MB. Heat shock proteins and thermoresistance in lizards// Proc Natl Acad Sci USA, 1992. V 89, P 1666 1670
- Viera J, Viera C. P, Hartl D. L, Losovskaya E.R. Factors contributing to the hybrid dysgenesis syndrome in Drosophila virilisll Genet Res, 1998. V 71, P 109−117
- Vogel JL, Parsell DA, Lindquist S. Heat-shock proteins Hspl04 and Hsp70 reactivate mRNA splicing after heat inactivation// Curr Biol. 1995, V 5(3), P 306−17
- Weber JA, Taxman DJ, Q. Lu and Gilmour DS. Molecular architecture of the hsplO promoter after deletion of the TATA box or the upstream regulation regionII Molecular and Cellular Biology, 1997. V17, P 3799 3808
- Wu C. Heat Shock Transcription Factors: Structure and Regulation// Annual Review of the Cellular and Development Biology, 1995. V 11, P 441 469
- Xing, H. Y., D. С. Wilkerson, С. N. Mayhew, E. J. Lubert, H. S. Skaggs et al. Mechanism of hsplOi gene bookmarking //Science, 2005. V 307, P 421−423
- Xu Y, Lindquist S. Heat-shock protein hsp90 governs the activity of pp60v-src kinase// Proc. Natl. Acad. Sci. USA, 1993. V 90 (15), P 7074 7078
- Yang S.-H., Hussenzweig A., Li L. et al. Modulation of thermal induction of hsplO expression by Ku autoantigen or its individual subunits// Mol. Cel. Biol., 1996. T 16, V 7, P 3799−3806
- Yanhong Shi, Morimoto R. Molecular chaperones as HSF 1-specific transcriptional repressors// Genes and Development, 1998. T 12, P 654 666
- Zelentsova E., Poluectova PI., Mnjoian L., Lyozin G., Veleikodvorskaja V., Zhivotovsky L., Kigwell M.G., Evgen’ev MB. Distribution and evolution of mobile elements in the virilis species group of Drosophilall Chromosoma, 1999. V 108 (7), P 443 456
- Zhang, P., and A. C. Spradling. Efficient and dispersed local-element transposition from Drosophila females// Genetics, 1993. V 133, P 361−373
- Zhong M., Orosz, A. and Wu, C. Direct sensing of heat and oxidation by Drosophila heat shock transcription factor// Mol. Cell, 1998. V 2, P 101−108
- Zhong M., Kim, S.-Y. and Wu, C. Sensitivity of Drosophila heat shock transcription factor to low pH// J. Biol. Chem., 1999. V 274, P 3135−3140